Three SNPs within exons of INHA and ACVR2B genes are significantly associated with litter size in Dazu black goats

The aim of this study was to analyse the association between single-nucleotide polymorphisms within INHA and ACVR2B and litter size in Dazu black goats. In total, twenty-two SNPs were genotyped in 190 individuals by SNaPshot and resequencing. The results showed that three SNPs (SNP_1, SNP_12 and SNP_13 in this study) were detected to have significant additive genetic effect on the recorded goat litter size (p < .05). The SNP_1 (NC_030809.1), a non-synonymous substitution of G for T at chr2-g. 28314990 in the exon 2 of INHA gene (NM_001285606.1), resulted in homozygote 2 (HOM2) contributed 0.25 and heterozygote (HET) contributed 0.12 larger litter than homozygote 1 (HOM1). Meanwhile, SNP_12 (Chr22-g. 11721225 A > T) and SNP_13 (Chr22-g. 11721227 A > C) (NC_030829.1) simultaneously mutated at the first and third position of a triplet AAA (lysine, K) in the exon 4 of ACVR2B gene (XM_018066623.1) had estimated genetic effects of HOM1 (0.00) and HOM2 (0.03) larger than HET (−0.12). In conclusion, one SNPs (chr2-g. 28314990 T > G) within the exon 2 of INHA and two SNPs (Chr22-g. 11721225 A > T and Chr22-g. 11721227 A > C) i n the exon 4 of ACVR2B gene were highly recommended as candidate markers of litter size in Dazu black goats. A large-scale association study to assess the impact of these variants on litter size is still necessary.     

Seasonal Losses of Surface Litter in Northern Great Plains Mixed-Grass Prairies

Surface litter protects rangeland soils against wind and water erosion and provides food and nesting materials for wildlife and insects. However, the ability of grassland systems to provide these services depends on the little studied topic of seasonal surface litter decomposition. Seasonal and annual surface litter decomposition rates were determined between 2014 and 2015 in central and western South Dakota at three mixed-grass prairie locations. Residue bags containing surface litter were placed in the field in late fall (1 November) of 2014 and removed after the winter (1 April), spring (1 July), and summer + fall seasons (1 November) of 2015. The litter was analyzed for total C, total N, acid detergent fiber (ADF), and acid detergent lignin (ADL). Average winter temperatures ranged from −5^oC to −15^oC, while summer temperatures ranged from 10^oC to 35^oC. Litter decomposition was lowest during the winter (0.57−0.86 g [kg × day]⁻¹) and greatest during the summer + fall (2.12−2.69 g [kg × day]⁻¹). Over the entire season, 40.8−62% of the surface litter decomposed. Winter litter decomposition was positively correlated with air temperature (r = 0.62, P < 0.01) and snow depth (r = 0.61, P < 0.01), and negatively correlated with C/N ratio (r = −0.65, P < 0.01), ADF (r = −0.35, P < 0.05), and ADL (r = −0.25, P < 0.05) concentrations. These findings indicate that winter decomposition cannot be ignored and that winter surface litter decomposition increases with snow depth.     

猪血浆转铁蛋白与部分经济性状关系的研究——Ⅰ. 多态性与产仔数和日增重的关系

用经改进的聚丙烯酰胺凝胶电泳铁染法,测定了二花脸猪血浆转铁蛋白多态类型,分析了其基因型频率和基因频率,并探讨了猪血浆转铁蛋白多态性与产仔性能和增重速度的相关性。     

羊草草原枯枝落叶中能量特征 分析

在草原生态系统中，以枯枝落叶为主体的能量的输入，积累和释放，是能量流动的主要环节。每年因植物的枯死，转移到枯枝落叶的能量为３９４９．６７９ＫＪ．ｍ６２．ａ，其季节变化呈指数形式。现存枯枝落叶中贮存的能量为３４２４．１０ＫＪ．ｍ＾２，经１年分解后，残留在枯枝落叶的能量为２２８２．４３ＫＪ．ｍ＾２，释放出的能量为１１４１．６０ＫＪ．ｍ＾２，能量释放的季节动态呈Ｓ型曲线。     

滇东北4种典型筇竹林凋落物的持水性

以滇东北4种典型筇竹林为研究对象，对比分析不同类型筇竹林凋落物储量及持水性能，从而为滇东北筇竹林生态可持续经营提供理论依据和实践指导。研究结果表明，凋落物储量为人工筇竹-黄皮树混交林（7.61 t/hm²） > 天然筇竹-人工黄皮树混交林（6.61 t/hm²） > 天然筇竹-人工厚朴混交林（5.73 t/hm²） > 天然筇竹纯林（5.23 t/hm²）。凋落物最大持水量为天然筇竹-人工厚朴混交林（3.45 t/hm²） > 人工筇竹-黄皮树混交林（3.22 t/hm²） > 天然筇竹-人工黄皮树混交林（2.89 t/hm²） > 天然筇竹纯林（2.69 t/hm²）。4种类型筇竹林凋落物吸水速率均随着浸泡时间延长而逐渐趋于一致，筇竹混交林凋落物吸水速率高于筇竹纯林。凋落物的总有效拦蓄量为人工筇竹-黄皮树混交林（9.74 t/hm²） > 天然筇竹-人工厚朴混交林（8.95 t/hm²） > 天然筇竹-人工黄皮树混交林（7.73 t/hm²） > 天然筇竹纯林（6.23 t/hm²）。     

微生物群落构建对杉木凋落叶分解及其酶活性的影响

为了分析不同微生物群落构建对于凋落物分解及其相应酶活性的影响，选取从杉木叶分离获得的内生真菌与腐生真菌共同构建五种不同的群落（室内实验）。结果表明：在凋落物分解3个月后，5组群落中，Ao+A群落引起的失重率最为显著，在其分解过程中Af+A群落对于C1酶活影响最为突出；在凋落物分解6个月后，Af+A群落对于凋落物失重率的影响最显著，在凋落物分解过程中Mr+A群落与Lp+A群落分别对过氧化物酶及漆酶酶活性影响最明显。总得来说，微生物群落构建对凋落物分解过程产生了重要影响。     

茶树凋落叶浸提液对菘蓝生理生化的化感效应

为研究茶树凋落叶浸提液对菘蓝生长与生理生化的化感效应,以模拟自然条件下雨雾淋溶方式,采用不同浓度的茶树凋落叶浸提液(CK:0 mg·mL^-1、T1:6.25 mg·mL^-1、T2:12.5 mg·mL^-1、T3:25 mg·mL^-1 和T4:50 mg·mL^-1)处理菘蓝,测定其生长、抗氧化酶活性及其基因表达量、细胞膜损伤率以及丙二醛(MDA)、过氧化氢(H₂O₂)、渗透调节物质和次生代谢物含量的变化。结果表明,茶树凋落叶浸提液对菘蓝生长表现出“低促高抑”的浓度效应,与CK相比,T1对菘蓝生长具有一定的促进作用,而浸提液浓度超过菘蓝的耐受阈值时,会对其生长产生不利的影响。随着浸提液浓度的升高,可溶性糖和可溶性蛋白含量呈先上升后下降的趋势,而脯氨酸含量则持续增加。与CK相比,T1的MDA含量、细胞膜损伤率和H₂O含量差异不显著,而T3、T4则显著升高。茶树凋落叶浸提液对抗氧化酶活性及其基因表达有不同的影响,T1的过氧化物酶(POD)与抗坏血酸氧化物酶(APX)活性最高,T2的过氧化氢酶(CAT)活性最高,而T3、T4的4种抗氧化酶活性均显著低于CK。T1的Pod、Cat、Apx基因表达量最高,而T4则抑制了抗氧化酶基因的表达。此外,POD活性与其基因表达量呈显著正相关,而超氧化物歧化酶(SOD)、CAT和APX活性与其基因表达量的相关性不显著。不同浓度的茶树凋落叶浸提液对于菘蓝次生代谢物质积累的影响存在显著差异,低浓度茶树凋落叶浸提液对菘蓝生长、靛蓝和靛玉红含量积累有促进作用,而高浓度茶树凋落叶浸提液对总黄酮含量积累有一定的促进作用。本研究结果可为幼龄茶园中茶树-菘蓝复合种植提供理论参考。